Labryo IVF Laboratory

IVF Lab
After the eggs are retrieved, they are examined in the laboratory for quality and maturity. Mature eggs are then placed in an IVF culture medium and transferred to an incubator for fertilization by the sperm.
 
Fertilization can be accomplished by insemination, where motile sperm are placed together with the oocytes and incubated overnight or by intracytoplasmic sperm injection (ICSI), where a single sperm is directly injected into each matured egg. In the United States, ICSI is performed in about 60% of ART cycles. ICSI is usually performed when there is a likelihood of reduced fertilization as a result of poor semen quality or history of failed fertilization in prior IVF cycle. Overall, pregnancy and delivery rates with ICSI are similar to the rates seen with traditional IVF.
 
On the egg retrieval day, embryologists will talk to patients about the number of eggs that has been retrieved. The embryologists will also discuss the embryo culture plan with patients.

ICSI (Intra-Cytoplasmic Sperm Injection)

ICSI is one of the greatest advances in ART history in the past 20 years. Patients must keep in mind that only mature eggs can be inseminated. Insemination, whether it is natural or through ICIS, occurs in the afternoon of the retrieval day (Day 0). During the ICSI procedure, an egg is stabilized under the microscope with a special micro-holding pipette; and a single sperm is picked up with a small needle and injected directly into the egg. Fertilization will be evaluated after 18 to 22 hours. ICSI is recommended for male patients who had prior failure in fertilization experiences or significant anti-sperm bodies. ICSI has a significantly higher fertilization rate, which is why we recommend most of the patients to do ICSI.
 
A fertilization check will be done by the embryologists in the next day (Day 1). Patients will be notified by a phone call, email, or text message regarding the fertilization status in the afternoon.
blastocyst
 

Blastocyst Culture

We strongly suggest to culture all embryos to blastocysts before transferring. If the embryos become blastocysts during Day 5 – 7, the embryos would be frozen and ready for patient’s next cycle for a frozen embryo transfer (FET). Not all embryos can grow into blastocysts. Based on patient’s specific circumstances, an embryologist will discuss a particular plan for each individual patient. Our embryologists will make recommendations based on their many years of experience to ensure that all the patient’s decisions are well-informed and based on their best interest.

blastocyst

Preimplantation Genetic Testing for aneuploidy (PGT-A), Preimplantation Genetic Testing for Monogenic Disorder (PGT-M), Preimplantation Genetic Testing for Structural Rearrangement (PGT-SR)

Pre-implantation genetic testing is the screening of individual embryos for genetic disorders or gender determination during the IVF process. It is a procedure of removing a couple of cells from the blastocyst to evaluate the embryo’s genetic composition and determine which embryos will be optimal for embryo transfer. Our lab offers PGT-A, PGT-M, and PGT-SR. It is an optional service and we will provide all the information to discuss with you on whether it would be beneficial for your IVF cycles. This procedure is safe for embryos since the embryologists will only be carefully removing 3 to 4 cells from blastocyst stage embryo.

Biopsy PGT-A TESTING

In addition, PGT-A testing can potentially decrease abnormal pregnancy, abortion rates, as well as increase pregnancy rate per transfer. It also decreases time-to-pregnancy and increases treatment cost-effectiveness.

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Embryo/Egg Cryopreservation 

Labryo uses the most advanced methods of cryopreservation. The method allows embryos to be used at a later date. Most recent studies have shown that the frozen embryo transfer has a higher pregnancy rate than a fresh embryo transfer. Labryo offers both egg and embryo vitrification method. The vitrification process has a higher survival rate. In our center, the egg/embryo recover rate is around 98%.

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